• Vance, T.D.R., Ye, Q., Conroy, B., Davies, P.L. (2020). Essential role of calcium in extending RTX adhesins to their target. J Struct Biol X. 2020 Sep 8; 4: 100036. doi: 10.1016/j.yjsbx.2020.100036. PubMed: 32984811

  • Ye, Q., Eves, R., Campbell, R.L., Davies, P.L. (2020). Crystal structure of an insect antifreeze protein reveals ordered waters on the ice-binding surface. Biochem J. 2020 Sep 18; 477 (17): 3271-3286. doi: 10.1042/BCJ20200539. PubMed: 32794579

  • Graham, L.A., Boddington, M., Holmstrup, M., Davies, P.L. (2020) Antifreeze protein complements cryoprotective dehydration in the freeze-avoiding springtail Megaphorura arctica. Sci. Rep. 2020 Feb 20; 10(1): 3047. doi: 10.1038/s41598-020-60060-z. PubMed: 32080305

  • Kinrade, B., Davies, P.L., Vance, T.D.R. (2020) Bacterial sugar-binding protein as a one-step affinity purification tag on dextran-containing resins. Protein Expr Purif. 2019 Dec 26:105564. doi: 10.1016/j.pep.2019.105564. Epub 2019 Dec 26. PubMed: 31883939


  • Vance, T.D.R., Guo S., Assaie-Ardakany S., Conroy B., Davies, P.L.  (2019). Structure and functional analysis of a bacterial adhesin sugar-binding domain. PLoS One. 2019 Aug 8;14(8):e0221101. doi: 10.1371/journal.pone.0221101.

  • Tomalty, H.E, Graham, L.A., Eves,R., Gruneberg, A.K., Davies, P.L. (2019) Laboratory-scale isolation of insect antifreeze protein for cryobiology. Biomolecules (in press)

  • Vance, T.D.R., Bayer-Giraldi, M., Davies, P.L., Mangiagalli, M. (2019). Ice-binding proteins and the 'domain of unknown function' 3494 family. FEBS J 286, 855-873.

  • Guo, S., Vance, T.D.R., Stevens, C.A., Voets, I., Davies, P.L. (2019). RTX adhesins are key bacterial surface megaproteins in the formation of biofilms. Trends in Microbiology. Jan 15. pii: S0966-842X(18)30279-8. doi: 10.1016/j.tim.2018.12.003. PubMed: 30658900


  • Guo, S., Langelaan, D.N., Phippen, S.W., Smith, S.P., Voets, I.K., Davies, P.L. (2018) Conserved structural features anchor biofilm-associated RTX-adhesins to the outer membrane of bacteria. FEBS J. 2018 Mar 25. doi: 10.1111/febs.14441 [Epub ahead of print] PubMed: 29575515.

  • Rismani Yazdi, S., Nosrati, R., Stevens, C.A., Vogel, D., Davies, P.L., Escobedo, C. (2018) Magnetotaxis Enables Magnetotactic Bacteria to Navigate in Flow. Small. 2018 Feb;14(5). doi: 10.1002/smll.201702982. Epub 2017 Dec 4.  PubMed: 29205792.

  • Vance, T.D.R., Graham, L.A., Davies, P.L. (2018) An ice-binding and tandem beta-sandwich domain-containing protein in Shewanella frigidimarina is a potential new type of ice adhesin. FEBS J. 2018 Mar 2. doi: 10.1111/febs.14424 [Epub ahead of print] PubMed:  29498209.

  • Ye, Q., Campbell, R.L., Davies, P.L. (2018) Structures of human calpain-3 protease core with and without bound inhibitor reveal mechanisms of calpain activation. J Biol Chem. 2018 Jan 30. pii: jbc.RA117.001097. doi: 10.1074/jbc.RA117.001097 [Epub ahead of print] PubMed:  29382717.


  • Guo, S., Stevens, C.A. Vance, T. D.R., Olijve, L.L.C., Graham, L.A., Campbell, R.L., Yazdi, S.R.,  Escobedo, C., Bar-Dolev, M. Yashunsky, V., Braslavsky, I., Langelaan, D.N., Smith, S.P.,  Allingham, J.S.,  Voets, I. K., Davies P.L. (2017) Structure of a 1.5-MDa adhesin that binds its Antarctic bacterium to diatoms and ice. Sci Adv. 2017 Aug 9;3(8):e1701440. doi: 10.1126/sciadv.1701440. eCollection 2017 Aug PubMed:  28808685.

  • Stevens, C.A., Semrau, J., Chiriac, D., Litschko, M., Campbell, R.L., Langelaan, D.N., Smith, S.P., Davies, P.L., Allingham, J.S. (2017) Peptide backbone circularization enhances antifreeze protein thermostability. Protein Sci. 2017 Jul 10. doi: 10.1002/pro.3228. [Epub ahead of print] PubMed:  28691252

  • Oude Vrielink, A.S., Vance, T.D.R., de Jong A.M., Davies, P.L., Voets, I.K. (2017) Unusually high mechanical stability of bacterial adhesin extender domains having calcium clamps. PLoS ONE 2017 Apr 4;12(4):e0174682. doi: 10.1371/journal.pone.0174682. eCollection 2017 PubMed:  28376122.


  • Bar-Dolev, M., Bernheim, R., Guo, S., Davies, P.L., Braslavsky, I. (2016) Putting life on ice: Bacteria that bind to frozen water. RSC Interface. 2016 Aug;13(121). pii: 20160210. doi: 10.1098/rsif.2016.0210. PubMed: 27534698

  • Bar-Dolev, M., Braslavsky, I., Davies, P.L. (2016) Antifreeze protein function. Ann. Rev. Biochem. 85, 515-542. PubMed: 27145844

  • Basu, K., Campbell, R.L., Guo, S., Sun, T., Davies, P.L. (2016) Modeling repetitive, non-globular proteins. Protein Sci. 25 (5), 946-58 Feb 23. doi: 10.1002/pro.2907. [Epub ahead of print] Review. PubMed:26914323

  • Basu, K., Wasserman, S.S., Jeronimo, P.S., Graham, L.A., Davies, P.L. (2016) Intermediate activity of midge antifreeze protein is due to a tyrosine-rich ice-binding site and atypical ice plane affinity. FEBS J. 283 (8) 2016 Feb 20. doi: 10.1111/febs.13687. [Epub ahead of print] PubMed:26896764


  • Sun, T., Davies, P.L., Walker, V.K. (2015) Structural basis for the inhibition of gas hydrates by alpha-helical antifreeze proteins. Biophys. J. 109, 1698-1705. PubMed: 26488661

  • Stevens, C., Drori, R., Zalis, S., Braslavsky, I., Davies, P.L. (2015) Dendrimer-linked antifreeze proteins have superior activity and thermal recovery. Bioconjugate Chemistry. PubMed: 26267368

  • Basu, K., Graham, L.A., Campbell, R.L., Davies, P.L. (2015) Flies expand the repertoire of protein structures that bind ice. PNAS 112(3), 737-742. PubMed: 25561557


  • Sun,T., Feng-Hsu Lin, F-H., Campbell, R.L., Allingham, J.S., and Davies, P.L. (2014) An antifreeze protein folds with an interior network of over 400 semi-clathrate waters. Science, 343(6172), 795-8 PubMed: 24531972 

  • Vance, T.D.R., Olijve, L.L.C., Campbell, R.L., Voets, I.K., Davies, P.L., Guo, S. (2014) Ca2+-stabilized adhesin helps an Antarctic bacterium reach out and bind ice. Bioscience Reports 34(4). PubMed: 24892750

  • Davies, P.L. (2014) Ice-binding proteins: a remarkable diversity of structures for stopping and starting ice growth. Trends in Biochemical Sciences 39(11), 548-555. PubMed: 25440715


  • Graham L.A, Hobbs R.S, Fletcher G.L, Davies P.L. (2013) Helical antifreeze proteins have independently evolved in fishes on four occasions.  PLoS ONE 8(12), e81285 PubMed: 24324684

  • Guo, S., Garnham, C.P., Partha, S.K., Campbell, R.L., Allingham, J.S., Davies, P.L. (2013) Role of Ca2+ in folding the tandem β-sandwich extender domains of a bacterial ice-binding adhesion. FEBS Journal Ref. no.: FJ-13-0467.R1.PubMed: 24024640

  • Celik Y., Drori R., Pertaya-Braun N., Altan A., Barton T., Bar-Dolev M., Groisman A., Davies P.L., Braslavsky I. (2013) Microfluidic experiments reveal that antifreeze proteins bound to ice crystals suffice to prevent their growth. Proc Natl Acad Sci U S A. 110,1309-14 PubMed: 23300286


  • Guo, S., Garnham, C.P., Whitney J.C., Graham, L.A. and Davies, P.L. (2012) Re-evaluation of a bacterial antifreeze protein as an adhesin with ice-binding activity. PLoS ONE 7, e48805 PubMed: 23144980

  • Garnham C.P., Nishimiya Y., Tsuda S., Davies P.L. (2012) Engineering a naturally inactive isoform of type III antifreeze protein into one that can stop the growth of ice. FEBS Lett. 586(21), 3876-81. PubMed: 23017208

  • Campbell R.L., Davies P.L. (2012) Structure-function relationships in calpains. Biochem J. 447, 335-51 PubMed: 23035980

  • Middleton, A.J., Marshall, C.B., Faucher, F., Bar-Dolev, M., Braslavsky, I., Campbell, R.L., Walker, V.K. and Davies, P.L. (2012) Antifreeze protein from freeze-tolerant grass has a beta-roll fold with an irregularly structured ice-binding site. J. Mol. Biol. 416, 713-724 PubMed: 22306740


  • Garnham, C.P., Campbell, R.L., Walker, V.K and Davies, P.L. (2011) Novel dimeric β-helical model of an ice nucleation protein with bridged active sites. BMC Structural Biology 11, 36 PubMed: 21951648   Free PDF at BioMed Central

  • Lin, F.H.,Davies, P.L. and Graham, L.A. (2011) The Thr- and Ala-rich hyperactive antifreeze protein from inch worm folds as a flat silk-like β-helix. Biochemistry 50, 4467-4478. PubMed: 21486083

  • Garnham, C.P., Campbell, R.L. and Davies, P.L. (2011) Anchored clathrate waters bind antifreeze proteins to ice. PNAS 108, 7363-7367. PubMed: 21482800 PubMed Central: PMC3088597


  • Garnham, C.P,. Natarajan, A,. Middleton, A.J., Kuiper, M.J., Braslavsky, I., Davies, P.L. (2010) Compound ice-binding site of an antifreeze protein revealed by mutagenesis and fluorescent tagging. Biochemistry 49, 9063-9071. PubMed: 20853841

  • Mok, Y.F., Lin, F.H., Graham, L.A., Celik, Y. Braslavsky, I., Davies, P.L. (2010) Structural basis for the superior activity of the large isoform of snow flea antifreeze protein. Biochemistry 49, 2593-2603. PubMed: 20158269

  • Celik Y., Graham, L.A., Mok, Y.F., Bar, M., Davies, P.L., Braslavsky I. (2010) Superheating of ice crystals in antifreeze protein solutions. Proc Natl Acad Sci U S A. 107, 5423-5428. PubMed: 20215465


  • Garnham, C.P., Hanna, R.A., Chou, J.S., Low, K.E., Gourlay, K., Campbell, R.L., Beckmann, J.S. and Davies, P.L. (2009) Limb-girdle muscular dystrophy type 2A can result from accelerated autoproteolytic inactivation of calpain 3. Biochemistry 48, 3457-3467. PubMed: 19226146

  • Middleton, A.J., Brown, A.M., Davies, P.L. and Walker, V.K. (2009) Identification of the ice-binding face of a plant antifreeze protein. FEBS Lett. 583, 815-819. PubMed: 19185572

  • Ravulapalli, R., Campbell, R.L., Gauthier, S.Y., Dhe-Paganon, S. and Davies, PL. (2009) Distinguishing between calpain heterodimerization and homodimerization. FEBS J. 276, 973-982. PubMed: 19215300


  • Hanna, R.A., Campbell, R.L. and Davies, P.L. (2008) Calcium-bound structure of calpain and its mechanism of inhibition by calpastatin. Nature 456, 409-412. PubMed: 19020623

  • Graham, L.A., Ewart, K.V., Lougheed, S.C. and Davies, P.L. (2008) Lateral transfer of a lectin-like antifreeze protein gene in fishes. PLoS ONE 3, e2616. PubMed: 18612417

  • Garnham, C.P., Gilbert, J.A., Hartman, C.P., Campbell, R.L., Laybourn-Parry, J. and Davies, P.L. (2008) A Ca2+-dependent bacterial antifreeze protein domain has a novel β-helical ice-binding fold. Biochem J. 411, 171-180. PubMed: 8095937

  • Graham, L.A., Marshall, C.B., Lin, F.H., Campbell, R.L. and Davies, PL. (2008) Hyperactive antifreeze protein from fish contains multiple ice-binding sites. Biochemistry 47, 2051-2063. PubMed: 18225917

We are grateful to the Canadian Institutes of Health Research (CIHR), the Natural Sciences and Engineering Research Council (NSERC) and the Muscular Dystrophy Association (MDA) for funding our research.


Queen's University

Kingston Ontario Canada

© 2018  Peter L. Davies

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