2024

• Eves, R., Davies, P.L. (2024). Protein inclusion into ice can dissociate subunits. Protein Expression and Purification. 224:106576. doi: 10.1016/j.pep.2024.106576. PubMed: 39137878

• Graham, L.A., Davies, P.L. (2024). Fish antifreeze protein origin in sculpins by frameshifting within a duplicated housekeeping gene. FEBS Journal. doi: 10.1111/febs.17205. PubMed: 38923815

• Graham, L.A., Hansen, T., Yang, Y., Sherik, M., Ye, Q., Soares, B., Kinrade, B. Guo, S., Davies, P.L. (2024). Adhesin domains responsible for binding bacteria to surfaces they colonize project outwards from companion split domains. Proteins. 2024 Apr 9. doi: 10.1002/prot.26689. PubMed: 38591850

• Sherik, M., Eves, R., Guo, S., Lloyd, C.J., Klose, K.E., Davies, P.L. (2024). Sugar-binding and split domain combinations in repeats-in-toxin adhesins from Vibrio cholerae and Aeromonas veronii mediate cell-surface recognition and hemolytic activities. mBio, 2024 Feb 14;15(2):e0229123. doi: 10.1128/mbio.02291-23 PubMed: 38171003

2023

• Hansen, T., Lee, J.C., Reicher, N., Ovadia, G., Guo, S., Guo, W., Liu, J., Braslavsky, I., Rudich, Y., Davies, P.L. (2023). Ice nucleation proteins self-assemble into large fibres to trigger freezing at near 0 ℃. eLife Dec 18;12:RP91976. doi: 10.7554/eLife.91976. PubMed: 38109272

• Lloyd, C.J., Guo, S., Kinrade, B., Zahiri, H., Eves, R., Syed, K.A., Yildiz F, Voets, I.K., Davies, P.L., Klose, K.E. (2023). A peptide-binding domain shared with an Antarctic bacterium facilitates Vibrio cholerae epithelial cell binding, biofilm formation and intestinal colonization. PNAS. 120(39):e2308238120. doi: 10.1073/pnas.2308238120. PubMed: 37729203

• Scholl, C.L., Holmstrup, M., Graham, L.A., Davies, P.L. (2023). Polyproline type II helical antifreeze proteins are widespread in Collembola and likely originated over 400 million years ago in the Ordovician Period. Scientific Reports. 2023 Jun 1;13(1):8880. doi: 10.1038/s41598-023-35983-y PubMed: 37264058

• Tomalty, H.E., Graham, L.A., Walker, V.K., Davies, P.L. (2023). Chilling injury in human kidney tubule cells after subzero storage is not mitigated by antifreeze protein addition. Cryobiology. 2023 Jun; 111:113-120. doi: 10.1016/j.cryobiol.2023.05.002. PubMed: 37164251

2022

• Scholl, C.L., Davies, P.L. (2022). Protein engineering shows antifreeze activity scales withice-binding site area. FEBS Lett. 597(4):538-546. doi:10.1002/1873-3468.14552 PubMed: 36460826

• Forbes, J., Bissoyi, A., Eickhoff, L., Reicher, N., Hansen, T., Bon, C.G., Walker, V.K., Koop, T., Rudich, Y., Braslavsky, I,, Davies, P.L. (2022). Water-organizing motif continuity is critical for potent ice nucleation protein activity. Nature Communications. 13(5019): 1-14. DOI: 10.1038/s41467-022-32469-9 PubMed: 36028506

• Graham, L. A., Gauthier, S.Y., Davies, P.L. (2022). Origin of an antifreeze protein gene in response to Cenozoic climate change. Scientific Reports. 12(8536): 1-16. doi: 10.1038/s41598-022-12446-4 PubMed: 35595816

• Davies, P.L. (2022). Reflections on antifreeze proteins and their evolution. Biochemistry and Cell Biology 100(4): 282-291 doi: 10.1139/bcb-2022-0029 PubMed: 35580352

​​2021

• Guo. S., Vance, T.D.R., Zahiri, H., Eves, R., Stevens, C., Hehemann, J.H., Vidal-Melgosa, S., Davies, P.L. (2021). Structural basis of ligand selectivity by a bacterial adhesin lectin involved in multispecies biofilm formation. mBio. 2021 April 6; 12(2): e00130-21. doi: 10.1128/mBio.00130-21. PubMed: 33824212

• Graham, L.A., Davies, P.L. (2021). Horizontal gene transfer in vertebrates: A fishy tale. Trends in Genetics. 2021 March 10; 37(6): 501-503. doi: 10.1016/j.tig.2021.02.006. PubMed: 33714557

• Gruneberg, A.K., Graham, L.A., Eves, R., Agrawal, P., Oleschuk, R.D., Davies, P.L. (2021). Ice recrystallization inhibition activity varies with ice-binding protein type and does not correlate with thermal hysteresis. Cryobiology. 2021 Jan 30; 99: 28-39. doi: 10.1016/j.cryobiol.2021.01.017. PubMed: 33529683

• Scholl, C.L., Tsuda, S., Graham, L.A., Davies, P.L. (2021). Crystal waters on the nine polyproline type II helical bundle springtail antifreeze protein from Granisotoma rainieri match the ice lattice. FEBS J. 2021 Jan 18; Online ahead of print. doi: 10.1111/febs.15717. PubMed: 33460499

2020

• Vance, T.D.R., Ye, Q., Conroy, B., Davies, P.L. (2020). Essential role of calcium in extending RTX adhesins to their target. J Struct Biol X. 2020 Sep 8; 4: 100036. doi: 10.1016/j.yjsbx.2020.100036. PubMed: 32984811

• Ye, Q., Eves, R., Campbell, R.L., Davies, P.L. (2020). Crystal structure of an insect antifreeze protein reveals ordered waters on the ice-binding surface. Biochem J. 2020 Sep 18; 477 (17): 3271-3286. doi: 10.1042/BCJ20200539. PubMed: 32794579

• Graham, L.A., Boddington, M., Holmstrup, M., Davies, P.L. (2020) Antifreeze protein complements cryoprotective dehydration in the freeze-avoiding springtail Megaphorura arctica. Sci. Rep. 2020 Feb 20; 10(1): 3047. doi: 10.1038/s41598-020-60060-z. PubMed: 32080305

• Kinrade, B., Davies, P.L., Vance, T.D.R. (2020) Bacterial sugar-binding protein as a one-step affinity purification tag on dextran-containing resins. Protein Expr Purif. 2019 Dec 26:105564. doi: 10.1016/j.pep.2019.105564. Epub 2019 Dec 26. PubMed: 31883939

2019

• Vance, T.D.R., Guo S., Assaie-Ardakany S., Conroy B., Davies, P.L.  (2019). Structure and functional analysis of a bacterial adhesin sugar-binding domain. PLoS One. 2019 Aug 8;14(8):e0221101. doi: 10.1371/journal.pone.0221101.

• Tomalty, H.E, Graham, L.A., Eves,R., Gruneberg, A.K., Davies, P.L. (2019) Laboratory-scale isolation of insect antifreeze protein for cryobiology. Biomolecules (in press)

• Vance, T.D.R., Bayer-Giraldi, M., Davies, P.L., Mangiagalli, M. (2019). Ice-binding proteins and the 'domain of unknown function' 3494 family. FEBS J 286, 855-873.

• Guo, S., Vance, T.D.R., Stevens, C.A., Voets, I., Davies, P.L. (2019). RTX adhesins are key bacterial surface megaproteins in the formation of biofilms. Trends in Microbiology. Jan 15. pii: S0966-842X(18)30279-8. doi: 10.1016/j.tim.2018.12.003. PubMed: 30658900

2018

• Guo, S., Langelaan, D.N., Phippen, S.W., Smith, S.P., Voets, I.K., Davies, P.L. (2018) Conserved structural features anchor biofilm-associated RTX-adhesins to the outer membrane of bacteria. FEBS J. 2018 Mar 25. doi: 10.1111/febs.14441 [Epub ahead of print] PubMed: 29575515.

• Rismani Yazdi, S., Nosrati, R., Stevens, C.A., Vogel, D., Davies, P.L., Escobedo, C. (2018) Magnetotaxis Enables Magnetotactic Bacteria to Navigate in Flow. Small. 2018 Feb;14(5). doi: 10.1002/smll.201702982. Epub 2017 Dec 4. https://doi.org/10.1002/smll.201702982  PubMed: 29205792.

• Vance, T.D.R., Graham, L.A., Davies, P.L. (2018) An ice-binding and tandem beta-sandwich domain-containing protein in Shewanella frigidimarina is a potential new type of ice adhesin. FEBS J. 2018 Mar 2. doi: 10.1111/febs.14424 [Epub ahead of print] PubMed:  29498209.

• Ye, Q., Campbell, R.L., Davies, P.L. (2018) Structures of human calpain-3 protease core with and without bound inhibitor reveal mechanisms of calpain activation. J Biol Chem. 2018 Jan 30. pii: jbc.RA117.001097. doi: 10.1074/jbc.RA117.001097 [Epub ahead of print] PubMed:  29382717.

2017

• Guo, S., Stevens, C.A. Vance, T. D.R., Olijve, L.L.C., Graham, L.A., Campbell, R.L., Yazdi, S.R.,  Escobedo, C., Bar-Dolev, M. Yashunsky, V., Braslavsky, I., Langelaan, D.N., Smith, S.P.,  Allingham, J.S.,  Voets, I. K., Davies P.L. (2017) Structure of a 1.5-MDa adhesin that binds its Antarctic bacterium to diatoms and ice. Sci Adv. 2017 Aug 9;3(8):e1701440. doi: 10.1126/sciadv.1701440. eCollection 2017 Aug PubMed:  28808685.

• Stevens, C.A., Semrau, J., Chiriac, D., Litschko, M., Campbell, R.L., Langelaan, D.N., Smith, S.P., Davies, P.L., Allingham, J.S. (2017) Peptide backbone circularization enhances antifreeze protein thermostability. Protein Sci. 2017 Jul 10. doi: 10.1002/pro.3228. [Epub ahead of print] PubMed:  28691252

• Oude Vrielink, A.S., Vance, T.D.R., de Jong A.M., Davies, P.L., Voets, I.K. (2017) Unusually high mechanical stability of bacterial adhesin extender domains having calcium clamps. PLoS ONE 2017 Apr 4;12(4):e0174682. doi: 10.1371/journal.pone.0174682. eCollection 2017 PubMed:  28376122.

2016

• Bar-Dolev, M., Bernheim, R., Guo, S., Davies, P.L., Braslavsky, I. (2016) Putting life on ice: Bacteria that bind to frozen water. RSC Interface. 2016 Aug;13(121). pii: 20160210. doi: 10.1098/rsif.2016.0210. PubMed: 27534698

• Bar-Dolev, M., Braslavsky, I., Davies, P.L. (2016) Antifreeze protein function. Ann. Rev. Biochem. 85, 515-542. PubMed: 27145844

• Basu, K., Campbell, R.L., Guo, S., Sun, T., Davies, P.L. (2016) Modeling repetitive, non-globular proteins. Protein Sci. 25 (5), 946-58 Feb 23. doi: 10.1002/pro.2907. [Epub ahead of print] Review. PubMed:26914323

• Basu, K., Wasserman, S.S., Jeronimo, P.S., Graham, L.A., Davies, P.L. (2016) Intermediate activity of midge antifreeze protein is due to a tyrosine-rich ice-binding site and atypical ice plane affinity. FEBS J. 283 (8) 2016 Feb 20. doi: 10.1111/febs.13687. [Epub ahead of print] PubMed:26896764

2015

• Sun, T., Davies, P.L., Walker, V.K. (2015) Structural basis for the inhibition of gas hydrates by alpha-helical antifreeze proteins. Biophys. J. 109, 1698-1705. PubMed: 26488661

• Stevens, C., Drori, R., Zalis, S., Braslavsky, I., Davies, P.L. (2015) Dendrimer-linked antifreeze proteins have superior activity and thermal recovery. Bioconjugate Chemistry. PubMed: 26267368

• Basu, K., Graham, L.A., Campbell, R.L., Davies, P.L. (2015) Flies expand the repertoire of protein structures that bind ice. PNAS 112(3), 737-742. PubMed: 25561557

2014

• Sun,T., Feng-Hsu Lin, F-H., Campbell, R.L., Allingham, J.S., and Davies, P.L. (2014) An antifreeze protein folds with an interior network of over 400 semi-clathrate waters. Science, 343(6172), 795-8 PubMed: 24531972 

• Vance, T.D.R., Olijve, L.L.C., Campbell, R.L., Voets, I.K., Davies, P.L., Guo, S. (2014) Ca2+-stabilized adhesin helps an Antarctic bacterium reach out and bind ice. Bioscience Reports 34(4). PubMed: 24892750

• Davies, P.L. (2014) Ice-binding proteins: a remarkable diversity of structures for stopping and starting ice growth. Trends in Biochemical Sciences 39(11), 548-555. PubMed: 25440715

2013

• Graham L.A, Hobbs R.S, Fletcher G.L, Davies P.L. (2013) Helical antifreeze proteins have independently evolved in fishes on four occasions.  PLoS ONE 8(12), e81285 PubMed: 24324684

• Guo, S., Garnham, C.P., Partha, S.K., Campbell, R.L., Allingham, J.S., Davies, P.L. (2013) Role of Ca2+ in folding the tandem β-sandwich extender domains of a bacterial ice-binding adhesion. FEBS Journal Ref. no.: FJ-13-0467.R1.PubMed: 24024640

• Celik Y., Drori R., Pertaya-Braun N., Altan A., Barton T., Bar-Dolev M., Groisman A., Davies P.L., Braslavsky I. (2013) Microfluidic experiments reveal that antifreeze proteins bound to ice crystals suffice to prevent their growth. Proc Natl Acad Sci U S A. 110,1309-14 PubMed: 23300286

2012

• Guo, S., Garnham, C.P., Whitney J.C., Graham, L.A. and Davies, P.L. (2012) Re-evaluation of a bacterial antifreeze protein as an adhesin with ice-binding activity. PLoS ONE 7, e48805 PubMed: 23144980

• Garnham C.P., Nishimiya Y., Tsuda S., Davies P.L. (2012) Engineering a naturally inactive isoform of type III antifreeze protein into one that can stop the growth of ice. FEBS Lett. 586(21), 3876-81. PubMed: 23017208

• Campbell R.L., Davies P.L. (2012) Structure-function relationships in calpains. Biochem J. 447, 335-51 PubMed: 23035980

• Middleton, A.J., Marshall, C.B., Faucher, F., Bar-Dolev, M., Braslavsky, I., Campbell, R.L., Walker, V.K. and Davies, P.L. (2012) Antifreeze protein from freeze-tolerant grass has a beta-roll fold with an irregularly structured ice-binding site. J. Mol. Biol. 416, 713-724 PubMed: 22306740

2011

• Garnham, C.P., Campbell, R.L., Walker, V.K and Davies, P.L. (2011) Novel dimeric β-helical model of an ice nucleation protein with bridged active sites. BMC Structural Biology 11, 36 PubMed: 21951648   Free PDF at BioMed Central

• Lin, F.H.,Davies, P.L. and Graham, L.A. (2011) The Thr- and Ala-rich hyperactive antifreeze protein from inch worm folds as a flat silk-like β-helix. Biochemistry 50, 4467-4478. PubMed: 21486083

• Garnham, C.P., Campbell, R.L. and Davies, P.L. (2011) Anchored clathrate waters bind antifreeze proteins to ice. PNAS 108, 7363-7367. PubMed: 21482800 PubMed Central: PMC3088597

2010

• Garnham, C.P,. Natarajan, A,. Middleton, A.J., Kuiper, M.J., Braslavsky, I., Davies, P.L. (2010) Compound ice-binding site of an antifreeze protein revealed by mutagenesis and fluorescent tagging. Biochemistry 49, 9063-9071. PubMed: 20853841

• Mok, Y.F., Lin, F.H., Graham, L.A., Celik, Y. Braslavsky, I., Davies, P.L. (2010) Structural basis for the superior activity of the large isoform of snow flea antifreeze protein. Biochemistry 49, 2593-2603. PubMed: 20158269

• Celik Y., Graham, L.A., Mok, Y.F., Bar, M., Davies, P.L., Braslavsky I. (2010) Superheating of ice crystals in antifreeze protein solutions. Proc Natl Acad Sci U S A. 107, 5423-5428. PubMed: 20215465

2009

• Garnham, C.P., Hanna, R.A., Chou, J.S., Low, K.E., Gourlay, K., Campbell, R.L., Beckmann, J.S. and Davies, P.L. (2009) Limb-girdle muscular dystrophy type 2A can result from accelerated autoproteolytic inactivation of calpain 3. Biochemistry 48, 3457-3467. PubMed: 19226146

• Middleton, A.J., Brown, A.M., Davies, P.L. and Walker, V.K. (2009) Identification of the ice-binding face of a plant antifreeze protein. FEBS Lett. 583, 815-819. PubMed: 19185572

• Ravulapalli, R., Campbell, R.L., Gauthier, S.Y., Dhe-Paganon, S. and Davies, PL. (2009) Distinguishing between calpain heterodimerization and homodimerization. FEBS J. 276, 973-982. PubMed: 19215300

2008

• Hanna, R.A., Campbell, R.L. and Davies, P.L. (2008) Calcium-bound structure of calpain and its mechanism of inhibition by calpastatin. Nature 456, 409-412. PubMed: 19020623

• Graham, L.A., Ewart, K.V., Lougheed, S.C. and Davies, P.L. (2008) Lateral transfer of a lectin-like antifreeze protein gene in fishes. PLoS ONE 3, e2616. PubMed: 18612417

• Garnham, C.P., Gilbert, J.A., Hartman, C.P., Campbell, R.L., Laybourn-Parry, J. and Davies, P.L. (2008) A Ca2+-dependent bacterial antifreeze protein domain has a novel β-helical ice-binding fold. Biochem J. 411, 171-180. PubMed: 8095937

• Graham, L.A., Marshall, C.B., Lin, F.H., Campbell, R.L. and Davies, PL. (2008) Hyperactive antifreeze protein from fish contains multiple ice-binding sites. Biochemistry 47, 2051-2063. PubMed: 18225917